| Product | Size | Kit Components | Product # |
|---|---|---|---|
| Agencourt AMPure XP 5 mL Kit | 139/278 preps1 | Agencourt AMPure XP Reagent | A63880 |
| Agencourt AMPure XP 60 mL Kit | 1,666/3,333 preps1 | Agencourt AMPure XP Reagent | A63881 |
| Agencourt AMPure XP 450 mL Kit | 12,500/25,000 preps1 | Agencourt AMPure XP Reagent | A63882
|
Application: PCR purification
Downstream Application Techniques: PCR, genotyping (SNP detection) fragment analysis, sequencing (Sanger and Next Generation), cloning, primer walking
Agencourt AMPure XP Features
High recovery of amplicons, greater than 100 bp Efficient removal of unincorporated dNTPs, primers, primer dimers, salts and other contaminants Stable PCR products post-cleanup: No PCR degradation after storage at 4° C for seven days Efficient recovery of double stranded and single stranded DNA templates Consistent recovery throughout the kit’s 12 month shelf life Faster manual and automated processing as compared to traditional post-PCR cleanup methodsProcess Overview
1. PCR reaction 2. Binding of PCR amplicons to magnetic beads 3. Separation of PCR amplicons bound to magnetic beads from contaminants 4. Washing of PCR amplicons with Ethanol 5. Elution of PCR amplicons from the magnetic particles 6. Transfer away from the beads into a new plate
High Recovery
Agencourt AMPure XP purification system recovers amplicons greater than 100 bp with consistency and high reproducibility. The recovery efficiency of the Agencourt AMPure XP kit for small and large amplicon sizes is superior to the traditional filtration PCR cleanup methods (Figure 1).
Figure 1. The recovery of the PCR products post-PCR cleanup was measured by spectrophotometry; 200 bp, 800 bp and 10 Kb PCR products wher purified with the Agencourt AMPure XP kit, QIAquick* kit and QuickStep* filtration system.
Walk-away Automated Solution
Agencourt AMPure XP post-PCR cleanup has been automated on three Beckman Coulter liquid handlers, the Biomek 3000, NXP and FXP for processing scalability. The LIMS compatible automation functionality is aided by a user-friendly interface equipped with a quick startup menu, a deck setup walk-through menu, a method recovery menu, and a procedure menu.
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